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Development of Lateral Flow Assay Based on Size-Controlled Gold Nanoparticles for Detection of Hepatitis B Surface Antigen
- Kim, Dong Seok;
- Kim, Yong Tae;
- Hong, Seok Bok;
- Kim, Jinwoon;
- Heo, Nam Su;
- ... Choi, Bong Gill;
- 외 5명
WEB OF SCIENCE
85SCOPUS
97초록
In this study, we developed lateral flow assay (LFA) biosensors for the detection of hepatitis B surface antigens using well-controlled gold nanoparticles (AuNPs). To enhance colorimetric signals, a seeded growth method was used for the preparation of size-controlled AuNPs with a narrow size distribution. Different sizes of AuNPs in the range of 342-137.8 nm were conjugated with antibodies and then optimized for the efficient detection of LFA biosensors. The conjugation stability was investigated by UV-vis spectroscopy of AuNP dispersion at various pH values and concentrations of antibody. Based on optimized conjugation conditions, the use of 42.7 +/- 0.8 nm AuNPs exhibited superior performance for the detection of LFAs relative to other sizes of AuNPs.
키워드
- 제목
- Development of Lateral Flow Assay Based on Size-Controlled Gold Nanoparticles for Detection of Hepatitis B Surface Antigen
- 저자
- Kim, Dong Seok; Kim, Yong Tae; Hong, Seok Bok; Kim, Jinwoon; Heo, Nam Su; Lee, Moon-Keun; Lee, Seok Jae; Kim, Byeong Il; Kim, In Soo; Huh, Yun Suk; Choi, Bong Gill
- 발행일
- 2016-12
- 유형
- Article
- 저널명
- Sensors
- 권
- 16
- 호
- 12
- 언어
- ENG
- 출판사
- MDPI
- 발행국가
- 스위스
- ISSN
- E 1424-8220