벼 조직배양 기간에 따른 retrotransposon(Tos17)의 활성에 관한 연구

초록

- Using the active-increment of Tos17copies in the genome of Oryza sativaL., there were many studies aboutinduction and selection of new mutants. This study mainly focuses on the induction for retrotransposon (Tos17) activityin the callus of Ilpumbyeo (Oryza sativaL.) according to varied culture period and condition. The objectives of thisstudy are obtaining various mutants (M1) through plant regeneration, identification of the mutation relation with Tos17,and subsequent phenotyping of the mutants (M2, M3). A total of 371 M1 mutants was obtained. The degree of Tos17activity obtained regeneration plants with each different culture period was evaluated by Southern blot analysis. Theresult showed that control Ilpumbyeo rice has 5 numbers of copies and the band numbers obtained 7, 8, 9.5, 12, 6, 13.5,17.5 from culture period of 1, 2, 3, 5, 6, 7, 8 month, respectively. In this study, the result showed that most effectualculture period for activity of Tos17in Ilpumbyeo rice is 5 month. Hereafter, collections and analysis of variousrecombination plants will act on an important factor in multiplication and preservation of M2 and M3 generation. And anurgent and important subject is a development of screening method for selection of diverse mutants.Key words - Tos17, Southern blot, Tissue culture, Rice, Mutant, Retrotransposon