Transient overexpression of the Miscanthus sinensis glucose-6-phosphate isomerase gene (MsGPI) in Nicotiana benthamiana enhances expression of genes related to antioxidant metabolism

초록

We investigated the expression of genes related to antioxidant mechanisms through transient overexpression of the glucose-6-phosphate isomerase (GPI) gene of Miscanthus sinensis in Nicotiana benthamiana leaves. Full-length cDNA was isolated from M. sinensis GPI (MsGPI). An analysis of the MsGPI amino acid sequence revealed significant similarities to the GPIs of Festuca ovima (94%), Hordium vulgare (94%), Triticum aestivum (94%), and Zea mays (97%). RT-PCR showed that MsGPI expression was induced significantly by NaCl. Transient M. sinensis GPI sense construct overexpression resulted in increased N. benthamiana ascorbate peroxidase (NbAPX) and phenylalanine ammonia lyase (NbPAL) transcript levels in N. benthamiana leaves. These observations suggest that MsGPI is involved in antioxidant metabolism and that it is a transcriptional regulator of NbAPX and NbPAL.

키워드