Degradation of 2,4,6-trinitrotoluene by Klebsiella sp. isolated from activated sludge

초록

Klebsiella sp. strain Cl isolated from activated sludge metabolized 2,4,6-trinitrotoluene (TNT) by two different pathways. The typical metabolites in the nitro group reduction pathway of TNT, such as hydroxylamino-dinitrotoluenes and amino-dinitrotoluenes, were detected. Dinitrotoluenes and nitrite were also detected, possibly produced by a denitration pathway. After incubation of [U-14C]TNT for 28 and 77 d, 2.4 and 6.24%, respectively, were released as 14CO<inf>2</inf>. This mineralization rate was higher than those reported by any other TNT degrading bacteria and might be due to the dual pathways of degradation in this bacterium.

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