Ethanol and supercritical fluid extracts of hemp seed (Cannabis sativa L.) increase gene expression of antioxidant enzymes in HepG2 cells

초록

Objective: To determine the gene expression of antioxidant enzymes by hemp seed extracts in human hepatoma (HepG2) cells. Methods: Ethanol and supercritical fluid (SF) extracts obtained from de-hulled hemp seed were used for the evaluation of in vitro antioxidant activity and gene expression of antioxidant enzymes. In vitro antioxidant activities of the samples evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) radical scavenging assays. The expression of antioxidant enzymes such as superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) in HepG2 cells was evaluated by real-time PCR. Results: In the antioxidant assay, SF extract of hemp seed exhibited higher ABTS and DPPH radical scavenging activities (IC<inf>50</inf> of 66.6 μg/mL and 9.2 mg/mL, respectively) than ethanol extract. The results of antioxidant enzyme expression in real-time PCR study revealed the H<inf>2</inf>O<inf>2</inf> (200 μM) challenged HepG2 cells reduced the expression of enzymes such as SOD, GPx and CAT. However, the cells treated with ethanol and SF extracts were up-regulated the expression of antioxidant enzymes in concentration dependent manner. When compared to ethanol extract, the SF extract exhibited higher activity in the expression of all the antioxidant enzymes at the concentration of 500 μg/mL. Conclusions: In conclusion, the findings of our study demonstrated that the hemp seed effectively inhibited H<inf>2</inf>O<inf>2</inf> mediated oxidative stress and may be useful as a therapeutic agent in preventing oxidative stress mediated diseases. © 2015 Hainan Medical College.

키워드