Utilization of Single Nodal Cuttings and Therapies for Eradicating Double-Infected Potato Virus (PLRV, PVY) from In Vitro Plantlets of Potato (Solanum tuberosum)

초록

In vitro plantlets of potato genotype F9-99 infected with PLRV and PVY were used for the virus eradication using heat and chemical treatments. Subculture medium was added with acetylsalicylic acid (ASA) (10-6, 10-5, and 10-4 M) and ribavirin (10, 15, and 20 mgL-1) alone or in combinations. The plantlets were incubated at 25C (control), and alternating heat treatments 35/30C for 1, 4, and 8 h or 42/25C for 4 h. Virus status of the plantlets were examined by means of double antibody sandwich-enzyme linked immunosorbent assay and reverse transcription polymerase chain reaction. After 48 weeks of treatment, 40, 51, 41, 36, and 0% survival were observed in the control (MS and 25C), ASA plus ribavirin (25C), MS plus heat treatment (35/30C), ASA plus ribavirin (35/30C), and MS medium with heat treatment (42/25C), respectively. As for virus eradication, heat treatment (35/30C) with 1 h alternating thermo-cycle resulted in 100% PVY elimination, but it was much more difficult to remove the PLRV from the double-infected potato plantlets with PVY and PLRV. The highest rate of PLRV eradication was 60% from the combined treatment of 10-5 M ASA, 20 mgL-1 ribavirin, and heat treatment at 35/30C for 8 h alternating temperature.

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