Characterization of Phage Behaviors Against Antibiotic-Resistant Salmonella Typhimurium

초록

This study was designed to investigate the dynamic behaviors of phages against Salmonella entericasubsp. enterica serovar Typhimurium ATCC 19585 (STWT), S. Typhimurium KCCM 40253 (STKCCM), ciprofloxacin-induced S. Typhimurium ATCC 19585 strains (STCIP), and S. Typhimurium CCARM 8009 (STCCARM). Phages,including PBST-10, PBST-13, PBST-32, PBST-35, P-22, and P-22 B1 had narrow host ranges. The adsorption rates ofall phages ranged from 47 to 85%, 58 to 95%, and 61 to 93%, respectively, against STWT, STKCCM, and STCIP, while thelowest adsorption rates ranged from 14 to 36% against STCCARM. The phage burst sizes were from 43 to 350, 37 to 530,66 to 500, and 24 to 500 plaque-forming units (PFUs) per infected STWT, STKCCM, STCIP, and STCCARM, respectively. TheSTCIP strain was effectively inhibited by all phages at the early of incubation period. These results provide useful informationfor better understanding the phage behaviors against antibiotic-resistant and antibiotic-sensitive pathogens.

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