Characterization of the New Cucumber Mosaic Virus Isolated from Atractylodes macrocephala

초록

Atractylodes macrocephala is a medicinal plant belonging to the genus Atractylodes, and it is widely cultivated in China and South Korea. To date, five viruses have been reported to infect A. macrocephala. In this study, A. macrocephala plants showing distortion symptoms were collected in Chuncheon, South Korea, followed by RT-PCR analysis using primer sets specific to these five viruses as well as primer sets targeting seven commonly occurring virus genera in South Korea. RT-PCR result revealed an amplicon of the expected size only with Cucumovirus-specific primers, and sequence analysis of the amplified product confirmed infection by cucumber mosaic virus (CMV). The newly identified isolate was designated CMV-Atm. CMV isolates from A. macrocephala have previously been reported in both China and South Korea. Therefore, the biological and molecular characteristics of CMV-Atm were compared with those of previously reported A. macrocephala-derived CMV isolates. CMV-Atm exhibited distinct pathogenicity and host responses compared with the previously identified isolates, although its phylogenetic analysis indicated high sequence similarity with them. Collectively, these findings suggest that CMV-Atm is genetically conserved among other A. macrocephala-infecting CMV isolates but displays unique symptom expression, implying subtle adaptations in its host-pathogen interactions.

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