Pap1-mediated regulation of thioredoxin gene from Schizosaccharomyces pombe

초록

The genomic DNA encoding thioredoxin (TRX) was previously isolated from the fission yeast Schizosaccharomyces pombe. In this investigation, regulation of the S. pombe TRX gene was studied in lacZ translational fusions. The synthesis of β-galactosidase from the fusion plasmid pYKT24 was significantly enhanced by treatments with cadmium chloride, zinc chloride, and high temperatures. Synthesis of β-galactosidase from the fusion plasmid was significantly decreased by higher concentrations (5 μM, 10 μM) of mercuric chloride, whereas it was enhanced by its lower concentration (1 μM). Diamide affected the synthesis of β-galactosidase in the same manner with mercuric chloride. However, high osmolarity had no effect on the β-galactosidase synthesis from the fusion plasmid pYKT24. Various fusion plasmids were constructed to carry serially deleted upstream regions of the TRX gene. Pap1 mediates the regulation of the S. pombe TRX gene. The upstream region, between 987 and 1,270 bp from the translational initiation point, is responsible for the regulation. ©KSMCB 2002.

키워드