젖소 초유로부터 TGF－β1의 정제

초록

Bovine colostrum contains various bio-functional proteins. Especially, transforming growth factor-β1 (TGF- β1) has a function in concerns with immune response. The purpose of this study is to establish the purification processing of TGF-β1. The highest concentration of TGF-β1 was measured within 48 h after parturition in bovine colostrum using ELISA kit. Purification of TGF-β1 from whey protein was carried out by the gel filtration, AF-heparin chromatography and AF-heparin rechromatography. After final purification step, TGF-β1 with a molecular weight of 25 kD was obtained, which were confirmed by silver staining and western blotting. Finally, TGF-β1 was identified native form of 25 kD and reducing form of 12.5 kD by reducing agent.

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