닭 전염성 기관지염을 검출하기 위한 합병혈청의 표본크기

초록

The sample sizes required to detect at least one chicken infectious bronchitis virus (IBV) infection at flock-level were determined using pooled samples for 48 submissions with different samples in each. A total of serum samplesof 9,980 layers from Kangwon, Chungpook and Chungnam province were collected and tested hemagglutinationinhibition (HI) antibody titers against IBV both individually and with pooling size of 10. Of the 48 submissions, 72.9%were required less than 5 pools to detect at least one infected pool at 95% confidence level, and the corespondingrate was 77.1% at 90% confidence level. Overall, the number of pools was decreased as the percent of positive poolsincreased. At two different cut-of HI titer. 9 and. 10 for individual samples the seroprevalence was 50.1% and 33.4%,respectively while 59.9% were seropositive for pooled samples at HI titer. and individual samples at each submission were 0.592 (p. 0.001) for HI titer. 9 and 0.561 (p. 0.001) for. 10, withcommon correlation coefficient of 0.576. This study indicated that pooled testing for the detection of IBV infection maybe an alternative strategy when only the pooled results are of interest and the prevalence has not known exactly.

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