백서 피질 신경원에서 N-methyl-D-aspartate로 유도된 뇌독성에 대한 Etomidate 효과

초록

Background: The present investigation was undertaken to evaluate the neuroprotective effect of etomidate against N-methyl-D- aspartate (NMDA) induced neurotoxicity in rat cortical neurons by measuring lactate dehydrogenase (LDH). Methods: The cerebral cortical neurons of fetal rat were grown for 12 days in dissociated cell culture. They were divided into four groups: first group acted as control with no drug administration and other groups treated with either NMDA 100μM or Etomidate (ET) 10μM + NMDA 100μM or ET 100μM + NMDA 100μM. After 24 hrs, cell death was assessed by morphology under the light microscope and quantified by measuring the LDH in the culture media. Results: In the light microscopic findings, the intact cortical neurons were increased in the ET groups. NMDA-induced LDH production also significantly suppressed in ET group (P ＜ 0.05). There were no significant difference between the ET 10μM and 100μM groups. Conclusions: These results suggest that the etomidate has protective effect on the cultured rat cortical neurons against NMDA-induced neurotoxicity.

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