Characteristics of asthmatics with detectable IL-32γ in induced sputum

초록

Background: Interleuldn-32(IL-32)gamma is a pro-inflammatory cytokine involved in the development and severity of chronic inflammatory diseases, but its role in asthma is unclear. Objective: This study was conducted to evaluate the relationship of IL-32 gamma levels in sputum with the severity of asthma. Methods: IL-32 gamma levels in the supernatant of induced sputum obtained from 89 patients with stable asthma were measured using a sandwich enzyme-linked immunosorbent assay (ELISA). The relationships between sputum IL-32 gamma levels and baseline forced expiratory volume in 1 s (FEV1% pred.), inflammatory cell profiles in sputum, and annual frequency of asthma exacerbation were determined. Results: IL-32 gamma was detected in the sputum of 25 of 89 (28.1%) asthma patients, and the levels of sputum were negatively correlated with FEV1% pred. (rho = 0.312, p = 0.003). The annual exacerbation rate was significantly higher in this group than in the IL-32-negative group (n = 64) (p = 0.03). Sputum IL-321 levels correlated well with the annual exacerbation rate (rho = 0.261, p = 0.014), but there were no differences in the inflammatory Cell profiles in the induced sputum of IL-32-positive and IL-32-negative patients. Conclusion: The level of IL-32 gamma in induced sputum may be associated with asthma severity and related with higher risk of asthma exacerbation. (C) 2017 Elsevier Ltd. All rights reserved.

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