Anti-oxidative and Neuroprotective Activities of Pig Skin Gelatin Hydrolysates

초록

This study was conducted to determine the antioxidative and neuroprotective effect of pig skin extracts (PS) and pig skin gelatin hydrolysates (LPS) using a human neuroblastoma cell line (SH-SY5Y). The extraction yield of PS was 3 fold higher than that of LPS. The protein content of PS was about 10 fold higher than that of LPS (p < 0.05). Also LPS increased antioxidative activity dose dependently, and the activity was significantly higher than PS at all concentration (p < 0.05). DPPH radical scavenging activity of LPS at 50 mg/mL was 92.97%, which was similar to 1 mu M vitamin C as a positive control. ABTS radical scavenging activity of LPS (20 mg/mL) was 89.83% and oxygen radical absorbance capacity of LPS at 1 mg/ mL was 141.39 mu M Trolox Equvalent/g. No significant change of human neuroblastoma cells was determined by MTT test. Cell death by oxidative stress induced by H2O2 and amyloid beta 1-42 (A beta(1-42)) was protected by LPS rather than PS. Acetylcholine esterase was significantly inhibited, by up to 33.62% by LPS at 10 mg/mL. Therefore, these results suggest that pig skin gelatin hydrolysates below 3 kDa have potential to be used as anti-oxidative and neuroprotective functional additives in the food industry, while further animal test should be determined in the future.

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