Rapid and efficient method for extraction and isolation of microcystin RR from the cyanobacterium

초록

A rapid and efficient method for extraction and isolation of microcystin RR from the cyanobacrerium is described. The method involves supercritical fluid extraction (SFE) for the fast extraction and silica gel column chromatography for the isolation of the microcystin RR. The procedure results in a purity of up to 96-98% microcystin RR without the need for a preparative HPLC. The advantage of the method developed here is that the sample handling steps are minimized, thus reducing possible losses of microcystin RR and saving extraction and isolation time.