엉겅퀴 추출물의 항상화성, 항돌연변이원성 및 항암활성 효과

초록

Cirsium japonicum var. ussuriense were extracted with methanol and then fractionated with n-hexane, EtOAc and BuOH to get active fractions. And their antioxidant and antimicrobial activities in each fraction were determined. Ethyl acetate and butanol fraction of Cirsium japonicum var. ussuriense showed strong antioxidant activities. but hexane fraction did not show any activities. But in the antimicrobial test, Ethyl acetate fraction showed strong antimicrobial activities except to Aspergillus awamori, Asperigillus niger. Especially, Ethly acetate fraction showed the strongest activities against Bacillus subtilis. And aqueous fraction showed the strongest activities against Cladosporium herbarum, Hypocrea nigricans. This study was performed to determine the antimutagenic and cytotoxic effect of Cirsium japonicum vat. ussuriense methanol extract on Salmonella typhimurium TA98, TA100 and cancer cell lines suing Ames test and cytotoxicity assay, respectively. Cancer cell lines include human lung carcinoma(a549), human breast adenocarcinoma(MCF-7) and human hepatocellular carcinoma (Hep3B). Futher fractionations with hexane, ethyl acetate, butanol and water from methanol extract of Cirsium japonicum var. ussuriense were performed to obtain effective fraction, methanol extract showed 60.4% inhibitory effect on the mutagenesis induced by MNNG against TA100, while 77% and 72.5% inhibition was observed on the mutagenesis induced by 4NQO against TA98 and TA100, respectively. and methanol extract showed 83.25% and 73.7% inhibitory effect on the mutagenesis induced by T게-P-1 against TA98 and TA100, respectively. methanol extract showed the strongest effect against A549,MCF-7 and H데3B at the same concentration compared to those of other fration.

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