β-Glucan enhanced apoptosis in human colon cancer cells SNU-C4

초록

The apoptotic effect of bacteria-derived beta-glucan was investigated in human colon cancer cells SNU-C4 Using terminal deoxynucleotidyl transferase (UT)-mediated dUTP nick end labeling (TUNEL) assay. reverse transcription-polymerase chain reaction (RT-PCR) expressions of Bcl-2, Bax, and Caspase-3 genes, and assay of caspase-3 enzyme activity. beta-Glucan of 10, 50 and 100 mu g/mL decreased cell viability in a dose-dependent manner with typical apoptotic characteristics, Such as morphological changes of chromatin condensation and apoptotic body formation from TUNEL assay. In addition, beta-glucan (100 mu g/mL) decreased the expression of Bcl-2 by 0.6 times, whereas the expression of Bax and Caspase-3 were increased by 3.1 and 2.3 times, respectively, compared to untreated control group. Furthermore, the caspase-3 activity in the beta-glucan-treated group, was significantly increased compared to those in control group (P<0.05). Bacterial derived beta-glucan could be used as all effective compound inducing apoptosis in human colon cancer.

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