Utilisation of spontaneous emulsification to fabricate lutein-loaded nanoemulsion-based delivery systems: factors influencing particle size and colour

초록

Factors influencing the formation and properties of lutein-loaded nanoemulsions fabricated using spontaneous emulsification (SE) were investigated. Nanoemulsion formation depended on oil type: small droplets (diameter approximate to 200nm) with a narrow monomodal particle size distribution (polydispersity index approximate to 0.23) could be formed using medium-chain triglycerides (MCT), but not long-chain triglycerides. Nanoemulsion formation also depended on surfactant type and concentration, with Tween 80 being the most effective surfactant. Optimisation of lutein-loaded nanoemulsions formed by SE led to systems with a final composition of 10wt% oil phase (0.12wt% lutein + 9.88wt% MCT), 10wt% Tween 80, and 80wt% aqueous phase. The nanoemulsions were stable to droplet aggregation when stored at ambient temperature for up to 1month; however, some colour fading occurred due to lutein degradation. This study indicated the potential of nanoemulsion-based delivery system fabricated using a low-energy method for encapsulation and protection of lutein.

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