Inhibitory Effects of Cheonggukjang Mucoid on 3T3-L1 Adipocyte Differentiation

초록

Cheonggukjang mucoid is typically composed of levan and polygamma glutamate (γ-PGA). In this study, we investigated the effect of levan and γ-PGA isolated from Cheonggukjang on the differentiation of 3T3-L1 preadipocytes. At 200 µg/mL PL 64 [γ-PGA:levan (6:4)] significantly inhibited triglyceride accumulation in adipocytes by 23.7% compared to untreated adipocytes (P<0.05). PL64 treatment reduced the expressions of fat accumulation-related genes, including peroxisome proliferator-activated receptor γ (pparγ), CCAAT-enhancer-binding protein α (c/ebpα), sterol regulatory element-binding protein-1c (srebp-1c), adipocyte protein 2 (ap2), fatty acid synthase (fas), acetyl-CoA carboxylase 1, and cluster of differentiation 36. In addition, PL64 reduced the protein levels of PPARγ, C/EBPα, SREBP-1C, AP2, FAS, and SCD1. These results show PL64 suppresses fat accumulation in vitro by down-regulating the expressions of adipogenesis-related genes and proteins. Consequently, this study shows that Cheonggukjang mucoid could be used as a potent anti-obesity functional substance that down-regulates fat accumulation-related genes and proteins.

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